Spatial and temporal evaluation of iodine uptake and radiodensity in meniscus tissue using contrast-enhanced micro-CT.

Rationale And Objective The visualization of soft tissues, like the meniscus, through X-ray micro-computed tomography (micro-CT), requires the use of contrast agents (CAs). While other studies have investigated CA diffusion in fibrocartilagineous tissues, this work aimed to optimize iodine staining protocols for meniscal tissue that improve their visualization by micro-CT. Specific objectives included evaluating the diffusion of CAs within meniscal samples over time, assessing volume changes due to staining, and identifying the iodine ions absorbed by the tissue.Materials And Methods Water-based and PBS-based Lugol solutions (KI3) were used to stain sheep and pig menisci for 24 days. Samples were scanned using micro-CT at different time points (0, 1, 4, 8, 12, 16, 20, and 24 days) to monitor CA diffusion and volume changes. Micro-CT provided three-dimensional (3D) visualization of iodine distribution and quantification of volume changes and radiodensity in the menisci. Additionally, UV-visible spectroscopy (UV-vis) analyses were performed to determine the uptake of iodine ions by the meniscus.Results Results indicated volumetric shrinkage and increased radiodensity within the first days of staining, with diffusion primarily occurring from the periphery of the meniscus. UV-visible spectroscopy identified two iodide ions in the CA solution (I- and I3 -) and revealed a preferential absorption of the triiodide ion (I3 -).Conclusion This study demonstrated the utility of iodine-based CAs and micro-CT technique for visualizing and investigating the spatial and temporal iodine diffusion within the meniscal tissue of sheep and pigs. The findings of this study have important implications for using iodine-based CAs in imaging analyses of the meniscus and offer potentially valuable insights into the diffusion patterns of iodine in fibrocartilagineous tissues.