The SpyBLI cell-free pipeline for the rapid quantification of binding kinetics from crude samples

Accurate measurements of binding kinetics, encompassing equilibrium dissociation constant (KD), association rate (kon), and dissociation rate (koff), are critical for the development and optimisation of high-affinity binding proteins. However, such...

Publication Details

Journal
Apollo (University of Cambridge)
Published
2025-06-24
DOI
https://doi.org/10.17863/cam.119381
Primary Topic
Biochemical and Structural Characterization
Type
article
Field-Weighted Citation Impact
0.00
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article

The SpyBLI cell-free pipeline for the rapid quantification of binding kinetics from crude samples

Apollo (University of Cambridge)
Biochemical and Structural Characterization
article

The SpyBLI cell-free pipeline for the rapid quantification of binding kinetics from crude samples

article en

Abstract

Accurate measurements of binding kinetics, encompassing equilibrium dissociation constant (KD), association rate (kon), and dissociation rate (koff), are critical for the development and optimisation of high-affinity binding proteins. However, such...

Apollo (University of Cambridge)
Openalex Percentile: Top 54%
Biochemical and Structural Characterization
DOIOpen Access PDFOpenAlex
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The SpyBLI cell-free pipeline for the rapid quantification of binding kinetics from crude samples · Apollo (University of Cambridge) (2025) | TGRS Research Map | TGRS