Mcart1 knockout enhances the level of macrophage inflammatory response in sepsis model mice

Objective To investigate the effect of Mcart1 knockout on acute inflammation of macrophages in vitro and in vivo. Methods The Mcart1 knockout mice were used to establish a sepsis model induced by lipopolysaccharide(LPS), and the survival period was measured. Bone marrow derived macrophages(BMDM) of LPS-induced inflammation mice were cultured in DMEM high-glucose medium. The mRNA levels of M1 and M2 related cytokines of BMDM after LPS stimulation were detected by RT-qPCR. The expression level of inflammation-related cytokines in serum of sepsis mice was detected by ELISA. Results Compared with wild type mice(Mcart1flox/flox), the survival time length of sepsis in Mcart1 knockout mice(Mcart1Lyz2-Cre)was significantly shortened(P＜0.001). After inflammation, the mRNA level of M1-related cytokines was up-regulated in BMDM cells of Mcart1Lyz2-Cre mice(P＜0.05); The mRNA level of M1-related cytokines was down-regulated(P＜0.05); The expression of M1-related mediators in serum of sepsis Mcart1Lyz2-Cre mice was up-regulated(P＜0.01). Conclusions Mcart1 knockout can significantly raise the inflammatory response of macrophages and aggravate the pathological symptoms of sepsis mice.