Correlation of peripheral blood miR-452 and miR-221 levelswith urinary sepsis after percutaneous nephrolithotomy with holmium laser

Objective To explore the correlation between the expression of microRNA-452 (miR-452) and micro-RNA-221 (miR-221) in peripheral blood and post-operative urinary sepsis after percutaneous nephrolithotripsy (PCNL). Methods From January 2019 to June 2023, 92 patients with post-operative urinary sepsis after PCNL admitted to 909 Hospital of the Chinese People′s Liberation Army Joint Logistic Support Force were regarded as the disease group, 92 patients who underwent PCNL surgery during the same period without urinary sepsis were collected as control group. RT-qPCR was applied to detect the expression of miR-452 and miR-221 in peripheral blood; Logistic regression was applied to analyze the influencing factors of urinary sepsis after PCNL surgery; Receiver operating characteristic (ROC) curve was applied to evaluate the diagnostic efficacy of peripheral blood miR-452 andmiR-221 levels for post-operative urinary sepsis in PCNL patients. Results The time length of the surgical operation in disease group was longer than that in the control group, and the level of miR-452 and miR-221 in peripheral blood, were higher than those in the control group (P＜0.05). Peripheral blood miR-452, miR-221, procalcitonin (PCT), C-reactive protein (CRP), neutrophil to lymphocyte ratio (NLR), urine routine and surgical time were all influence factors for post-operative urinary sepsis in PCNL (P＜0.05). The area under the curve (AUC) for the combined diagnosis of peripheral blood miR-452 and miR-221 in patients with urinary sepsis after PCNL surgery was 0.888, which was better than their individual detection (Zcombination-miR-452=2.005, Zcombination-miR-221=2.972, P=0.045, 0.003), the sensitivity and specificity were 77.17% and 91.30%, respectively. Conclusions The change of miR-452 and miR-221 level in peripheral blood is closely related to urinary sepsis after PCNL. Combined testing has a high diagnostic efficacy for post-operative urinary sepsis after PCNL surgery.