Knockdown strategy for <i>Tg</i>ATM1 and <i>Pf</i>ATM1.

<p>(<b>A</b>) Cartoon scheme showing the principle of the conditional downregulation strategy employed for <i>Tg</i>ATM1 based on dimerizable Cre recombinase (DiCre) and mRNA destabilization. The U1-recognition site moves closer to the stop codon upon activation of the DiCre and causes destabilization and degradation of the mRNA resulting in reduced expression of <i>Tg</i>ATM1. (<b>B</b>) Genomic integration PCR probing the parental line (DiCreU1) and <i>Tg</i>ATM1-Ty-U1 parasites to validate the integration of the intended construct, including a Ty-tag, a U1 recognition site, loxP sites and a selection cassette, in the 3′UTR of <i>Tg</i>ATM1. The approximate primer binding sites are given in the scheme and primers are listed in <a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1012593#ppat.1012593.s011" target="_blank">S2 Table</a>.</p> <p>(PDF)</p>