Expression of the fused proteins. HEK 293T cells were transfected with scFv-MeCP2, scFv-MeCP2 T158M, scFv-MeCP2 delMBD, scFv-HP1, and scFv-HDAC1 (A) or scFv-LANA (B) expression vectors.
Cell extracts were subjected to SDS-PAGE and western blot analysis. The scFv-fused proteins (upper panel) and beta-actin (lower panel) were detected with anti-HA and anti-beta actin antibodies, respectively. (PDF)
Publication Details
- Journal
- Figshare
- Published
- 2025-03-10
- DOI
- https://doi.org/10.1371/journal.ppat.1012972.s001
- Primary Topic
- Mycorrhizal Fungi and Plant Interactions
- Type
- article
- Field-Weighted Citation Impact
- 0.00