Representative gating strategy to assess NK cell depletion in SCID mice.

<p>Spleens from SCID mice of untreated or treated with anti-Asiola-GM1 blocking antibody were harvested, and single-cell suspensions were prepared as outlined in the Materials and Methods. Cells were stained with antibodies against CD45, CD3, and CD49b. The gating strategy shown identifies NK cell populations (CD45+CD3-CD49b+) by excluding debris, focusing on single, live cells. Single-stained and unstained controls were used to define gating parameters and ensure accurate compensation.</p> <p>(DOCX)</p>