Spectralis FAM imaging on varied total sensitivity settings, with/without normalization function on phantom fluorescent probe (A, B) and on Cy0410 retina before (C, D) and after (E, F) HEA/3 nmol TcapQ488 intravitreal injection procedures. Numbers in

Spectralis FAM imaging on varied total sensitivity settings, with/without normalization function on phantom fluorescent probe (A, B) and on Cy0410 retina before (C, D) and after (E, F) HEA/3 nmol TcapQ488 intravitreal injection procedures. Numbers in images are total sensitivity setting for the image when acquired, respectively. When Spectralis normalization function was turned off, fluorescent signal intensity detected from the phantom and its background corresponded with the total sensitivity setting, i.e., higher total sensitivity detected higher background intensity and fluorescent signal intensity (background signal subtracted) (A, G). When Spectralis normalization function was turned on, both background intensity and phantom fluorescent signal intensity were clamped at respective system defined signal and background optimal intensity regardless of the total sensitivity settings (B, G). During in vivo FAM imaging in the eyes of Cy0410 prior procedures HEA/intravitreal