<i>lcrF</i> transcript detection.

<p><i>Y</i>. <i>pseudotuberculosis</i> (YPIII), YP356 (Δ<i>rnc</i>), and YP139 (Δ<i>pnp</i>) were grown at 37°C, and the total RNA of the strains was prepared. 10 μg of total RNA of the different strains were loaded onto a denaturing acrylamide gel to allow the detection of smaller degradation products. The <i>lcrF</i> transcripts were identified by Northern blot using a probe covering the <i>lcrF</i> coding sequence. The blot represents one of two biological replicates. The 5 S rRNA was used as loading control.</p> <p>(PDF)</p>