Demonstration that HA- and FLAG-tagged Drosocin and Cecropin A1 can be detected by sandwich ELISA following bacterial infection.

<p>(A) Tagged Drosocin levels are significantly higher 3 hours after injection with PBS (n = 8; p = 0.0455) or ~6.0x10<sup>4</sup> cells of the Gram-negative bacterium <i>Enterobacter cloacae</i> (n = 8; p<0.001) than they are in uninjured flies (n = 8). <i>E</i>. <i>cloacae</i> infection results in significantly higher Drosocin levels than PBS injection (n = 11: p<0.001; linear model with Tukey post-hoc). (B) Tagged Cecropin levels are significantly higher 3-hours after infection with <i>E</i>. <i>cloacae</i> (n = 13) than after PBS injection (n = 11; p<0.001) or in uninjured flies (n = 8; p<0.001). There was no detectable difference in Cecropin levels between uninjured and PBS-injected flies (p = 0.32). Letters denote pairwise difference p<0.05.</p> <p>(DOCX)</p>