Replicate blots from the original study and repeat blots carried out in 2017.

Replicate blots (right-hand images) and later repeat blots (left-hand images). Page 1: Coomassie stain; Page 2: PAI-1 immunoblot (equivalent to Fig 2c); Page 3: Collagen I immunoblot (equivalent to Fig 2a; note a different antibody is used in the later repeat experiment); Page 4: TIMP-2 immunoblot (equivalent to Fig 4b). Arrows indicate the molecular weight for the target protein bands. Sample labels differ between the S1 File and the original published article; correlative labels are as follows: Het 24 LG = <i>db/wt</i> NG or non-diabetic cells treated with normal glucose media (NG; low glucose, LG) for 24 hours; Het 24 LG>HG = <i>db/wt</i> HG or non-diabetic cells treated with hyperglycemic (high glucose, HG) media for 24 hours; Db 24 HG = <i>db/db</i> HG or diabetic cells treated with hyperglycemic (high glucose, HG) for 24 hours; Db 24 HG>LG = <i>db/db</i> NG or diabetic cells treated with normal glucose media (NG; low glucose, LG) for 24 hours. (PDF)