Effects of ORF6 on RNA export in <i>Xenopus</i> oocytes.

<p>(A) Purified recombinant GST or GST-ORF6-His (50 fmol/oocyte) was pre-injected into the cytoplasm of <i>Xenopus</i> oocytes. After a 12-hour incubation, a mixture of <i>in vitro</i>-transcribed <sup>32</sup>P-labeled RNAs containing DHFR mRNA, pre-ftz mRNA, U6Dss snRNA, and tRNA<sup>Phe</sup> was injected into the nucleus. RNA was immediately extracted from nuclear (N) and cytoplasmic (C) fractions (T = 0) or 30 min after the injection, and then analyzed by 8% denaturing PAGE and autoradiography. Bands corresponding to the spliced product (spliced-ftz mRNA) and the lariat intron (intron) are indicated. (B) Quantification of the export of tRNA<sup>Phe</sup>. Values are the means (SD) (n = 3).</p> <p>(PDF)</p>