S2 Fig -

Loss of DLD GCVL-2 expression in the absence of hygromycin (Hyg). The pLPHyg-DLD plasmid was introduced into DLD-deficient L. major parasites in the logarithmic growth phase via electroporation. Transfected parasites were cultured in axenic media with (+) or without (-) hygromycin. At the indicated time points post-culture, the presence of the GCVL-2 DLD gene product (A) and its mRNA expression (B) was analyzed using PCR and RT-PCR, respectively. **, p <0.01; ****, p < 0.0001; ns, not significant. (DOCX)