S6 Fig -

<p>(A) Western blot of the whole cell lysates of the BAP-tagged GiMlf cell line with and without CytoBirA detecting the expression of BirA-HA with anti-HA antibody and the successful biotinylation of BAP-tagged GiMlf using streptavidin. (B) Western blot of the isolation process by biotin affinity purification of BAP-tagged GiMlf and its interaction partners. The ten-fold diluted supernatant of crosslinked proteins was incubated with the streptavidin coated beads. GiMlf-BAP was detected by an anti-BAP antibody. Lys–lysate, P–pellet, S–supernatant, dil–diluted, F–flowthrough, W–wash. (C) Localization of GiMlf and Tom40 in <i>Giardia</i> using expansion microscopy. The cells were stained with anti-BAP antibody (green) and anti-Tom40 antibody (magenta). A single layer is shown. Scale bars: 5 μm. (D) The presence of Hsp40 in the axonemes. The cells were stained with an anti-BAP antibody (green) and an anti-V5 antibody (magenta). Nucleic DNA was stained with DAPI (blue), DIC image of corresponding cell is shown in corner of the merged image. Scale bar: 2 μm. (E) Localization of BAP-tagged GiMlf (anti-BAP antibody, green) and its putative interaction partners isolated in the biotin affinity purification assay (anti-V5, magenta) by confocal fluorescence microscopy. DIC image of corresponding cell is shown in corner of the merged image. Scale bars: 2 μm.</p> <p>(PDF)</p>