Viable <i>in vitro</i>-differentiated bradyzoites do not infect Caco-2 in MPS.

(A) Fluorescent images showing no replication of <i>in vitro</i> differentiated ME49 mCherry bradyzoites in the Caco-2 lumen after 5- or 6- days post-infection. (B) Fluorescent images of ME49 mCherry parasites replicating in Caco-2 after 3 days post-infection, as a positive control of parasite viability. They infected as <i>in vitro</i> differentiated bradyzoites in MPS. (C) Determination of cell viability of <i>in vitro</i> differentiated bradyzoites after pepsin digestion by using SYTOX-green. (D) Caco-2 lumens infected with ME49 mCherry tachyzoites used as a control of infection. Punctuated lines represent the edges of the lumen and its boundary with the matrix. (PDF)