Immunogenicity of a headless HA+WT HI/19 vaccine in mice with pre-existing immunity, related to Fig 4.

(A–D) A pre-immune/WT group was immunized separately and the post-vaccination sera were compared to the sera collected from the three vaccine groups described in <a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1012878#ppat.1012878.g004" target="_blank">Fig 4</a>. Post-vaccination sera were used to detect antibody responses against whole HI/19 virus (A), HI/19 HA head (B), HI/19 HA stalk (C) and HI/19 NA (D) via ELISA. N = 5 mice. All experiments were performed at least two times and similar results were observed. For all panels, Mann-Whitney U tests were performed. Data shown as mean ± SEM. (DOCX)