Chromatograms from size-exclusion chromatography of recombinant <i>Naegleria fowleri</i> cystatin, lysozyme and hemerythrin.

The molecular weights calculated for each significant peak in the chromatograms are shown within tables. Molecular weights were calculated from calibration curves of the Gel Filtration Standards (Bio-Rad, USA) and for hemerythrin from Gel Filtration LMW Calibration Kit (Cytiva, USA). The peak of the standards along with their molecular weight is listed at the top of the chromatographs. The inset in each chromatograph shows SDS-PAGE of the recombinant protein. (PDF)