TRACS selects for essential genes based on the most sgRNAs.

<p>(A) Bar plot showing the distribution of the number of sgRNAs per gene for the 6,717 genes that had ER < 0 and <i>p</i><sub><i>adj</i></sub> < 0.05 in TRACS. Light blue color corresponds to light blue data points shown in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0315923#pone.0315923.g001" target="_blank">Fig 1D</a>. (B) Bar plots showing the distribution of sgRNAs per gene discovered by MAGeCK-RRA and MAGeCK-MLE with LFC < 0 and unadjusted <i>p</i> value < 0.05. Purple and green colors correspond to the colored data points in the volcano plots in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0315923#pone.0315923.s003" target="_blank">S3 Fig</a>. Most top-ranked genes identified by MAGeCK-RRA had 6 sgRNAs per gene although at reduced frequency which is attributed to fewer genes discovered by MAGeCK. MAGeCK-MLE had wider disparity across genes as it made essentiality calls using as low as 1 sgRNA per gene. The peaks at 4 sgRNAs per gene in each of the three histograms represent miRNAs which have a maximum of 4 sgRNAs instead of 6.</p> <p>(PDF)</p>