Whole-genome sequencing and FACS analysis of <i>far8</i>Δ, <i>pph22</i>Δ, and <i>mob3</i>Δ mutants.

<p>(A) Read depth analyses of YL99<b>a</b> wild-type and <i>far8</i>Δ mutants showed duplications of the majority of chromosomes in the mutants, similar to what was observed in H99 <i>far8</i>Δ strains. <i>far8</i>Δ<i>-5</i> appeared to have an additional copy of chromosome 6. (B) FACS analyses of <i>mob3</i>Δ, <i>pph22</i>Δ, and <i>far8</i>Δ strains to determine ploidy, compared to wild-type 1N (H99) and 2N (CnLC6683) controls. Cells were stained with propidium iodide and DNA amount was quantitatively measured. <i>mob3</i>Δ mutants are haploid, with two peaks resembling H99 (1N). <i>pph22</i>Δ isolates only show one peak, suggesting cell cycle defects. YL99<b>a</b> <i>far8</i>Δ isolates had peaks resembling the wild-type 2N control, suggesting they are diploid.</p> <p>(DOCX)</p>