Sequence alignment of the <i>mnuA</i> genes undergo frameshift mutations in <i>M</i>. <i>bovis</i> field strains.

<p>Nucleotide sequences of the <i>mnuA</i> genes were extracted from the genomes of <i>M</i>. <i>bovis</i> PG45 type strain (ATCC 25523 / <a href="http://www.hpacultures.org.uk/products/bacteria/detail.jsp?refId=NCTC+10131&collection=nctc" target="_blank">NCTC 10131</a>; accession no. NC_014760.1) and <i>M</i>. <i>bovis</i> field strains 4877 (<a href="https://www.ncbi.nlm.nih.gov/nuccore/WFEJ01000016" target="_blank">WFEJ01000016.1</a>), 514 (<a href="https://www.ncbi.nlm.nih.gov/nuccore/WFAW01000057" target="_blank">WFAW01000057.1</a>), 17DD0020 (<a href="https://www.ncbi.nlm.nih.gov/nucleotide/NZ_JASFAP010000001.1?report=genbank&log$=nuclalign&blast_rank=1&RID=8S86RGXN01N" target="_blank">NZ_JASFAP010000001.1</a>) and F9160 (accession no. CP092777.1). The multiple alignment was conducted using the CLUSTAL O (1.2.4) (<a href="https://www.ebi.ac.uk/Tools/msa/clustalo/" target="_blank">https://www.ebi.ac.uk/Tools/msa/clustalo/</a>). The identical nucleotides are marked with asterisk (*). The start and stop codons are bolded. The region with a frameshift is marked by red, bolded and framed.</p> <p>(DOCX)</p>