<i>T. gondii</i> infection in the jejunum of mice fed brain containing cysts at 5 days post-feeding.

(A) Confocal images of female and male jejunum showing <i>T. gondii</i> parasites replicating near areas with excessive cell infiltration. (B) Confocal image of tachyzoites replicating surrounded by CD45-positive cells. (C) 3D projection of tachyzoites replicating within the stroma. (D) Epifluorescent image of tachyzoites (red) near the endothelium. RBC states for red blood cells. (E) Representative epifluorescent images of uninfected jejunum stained against CD45. (F) and (G) Show CD31-positive cells, as a result of our best attempt to obtain CD31 in the jejunal villi after 3 days post-feeding. Intestines are stained for polyclonal antibodies against <i>T. gondii</i> (green), CD31 (red), CD45 (green), and nuclei (blue, DAPI). (PDF)