No change in GAD67<sup>+</sup> neurons in <i>Phf6</i>-deleted mice.

<p>(A) Representative sections of N = 3 <i>Phf6</i><sup><i>+/Y</i></sup><i>;Nes-cre</i><sup><i>Tg/+</i></sup> and 3 <i>Phf6</i><sup><i>lox/Y</i></sup><i>;Nes-cre</i><sup><i>Tg/+</i></sup> adult brains (13–14 weeks old) stained with anti-GAD67 antibody by immunohistochemistry. Scale bar equals 100 μm. The schematic on the right indicates the approximate locations where GAD67<sup>+</sup> cells were quantified in sections in the left and right hemisphere on each cortex section. (B) Enumeration of GAD67<sup>+</sup> cells per mm<sup>2</sup> of cortex section. The average ± sem for each genotype is shown with circles representing data from individual animals. The number of GAD67<sup>+</sup> cells was counted on matched sections of the parietal cortex and divided by the area analysed. Data were analysed by two-tailed Student’s t-test (p = 0.51).</p> <p>(PDF)</p>