Localization of PIP2 signals visualized by the combination of specific antibody and GST-tagged GST-PLCδ1 PH domain in U2OS cell nucleus.

<p><b>(A)</b> Representative images of immunofluorescence staining for PIP2 using specific antibody and anti-GST antibody to visualize the PLCδ1 PH domain signal show the colocalization of PIP2 in the nuclei of U2OS cells. Images were captured by confocal microscopy. Scale bars correspond to 5 μm. <b>B)</b> Statistical analysis of colocalization parameters by Pearson’s, Spearman’s and Manders’ coefficients M1 and M2 compared to random images was performed using Student’s t-tests. Error bars correspond to SEM (**** P < 0.0001), n = 3, N = 34 cells.</p> <p>(PDF)</p>