NDD mice loose Lgr5+ stem cells in the colon.

<p>(A) qPCR of gene expression of <i>Lgr5</i> on RNA extracted from distal colon of <i>N1+/+; N2+/+</i> and <i>N1RA/RA; N2RA/RA</i> mice treated for 10 days 1% DSS relative to controls, n = 3 mice per group. *<i>p</i> <0.05, **<i>p</i> <0.01, ***<i>p</i> <0.001. (B) Daily weight measurements of <i>N1+/+; N2+/+; Lgr5-EGFP-IRES-creERT2</i> or <i>N1RA/RA; N2RA/RA; Lgr5-EGFP- IRES-creERT2</i> mice treated with 2% DSS 5 days, all <i>N1RA/RA; N2RA/RA; Lgr5-EGFP-IRES-creERT2</i> mice had to be euthanized within 6 days due to severe colitis-induced weight loss. *<i>p</i> <0.05, **<i>p</i> <0.01, ***<i>p</i> <0.001. (C) Immunofluorescence staining of GFP and Cytokeratin 8+18 in distal colon. White dashed box indicated zoomed region. Scale bars = 50 μm. (D) Quantification of number of GFP-positive cells per crypt. n = 3 mice per group. *<i>p</i> <0.05, **<i>p</i> <0.01, ***<i>p</i> <0.001.</p> <p>(PDF)</p>