<i>cycΔ5</i> strains mate efficiently and Fus1N fusogenic activity is negligible at short time frames.

<p>(<b>A, B</b>) Mating efficiency (% of cell pairs among all cells; A) and fusion efficiency (% of paired cells that fused; B) of WT or <i>cycΔ5</i> cells after 24 h on plates lacking nitrogen. The cells were pre-grown on ammonium or glutamate-containing medium. (<b>C</b>) Fusion efficiency (% of paired cells that fused) of <i>h90 fus1</i><sup><i>opto</i></sup> cells starved and kept in the dark for 24 h (same data as on <a href="http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.3002963#pbio.3002963.g001" target="_blank">Fig 1D</a>) and of <i>h+</i> x <i>h- cycΔ5 fus1</i><sup><i>opto</i></sup> cells individually starved for 2 h and then mixed and kept in the dark for a further 2 h 30. The latter <i>h+</i> x <i>h- cycΔ5 fus1</i><sup><i>opto</i></sup> conditions are identical to those used in the (phospho)proteomic time course, corresponding to time 150 min in <a href="http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.3002963#pbio.3002963.g003" target="_blank">Fig 3A</a>. As these cells do not fuse, this confirms that all phosphorylation changes observed in the mating time course occur before gamete fusion. <i>T</i> test <i>p</i>-value is indicated. The underlying data can be found in <a href="http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.3002963#pbio.3002963.s013" target="_blank">S1 Data</a>.</p> <p>(PDF)</p>