The treatment with VPA, Li<sub>2</sub>CO<sub>3</sub>, and tranilast reduces DNA double strand breaks as indicated by γH2Ax but does not restore HMGB-1 levels.

(A) Immunofluorescent staining of non-senescent (NS), replicative senescent untreated (RS untr) and replicative senescent treated (RS treated) cells for DAPI (blue) and γH2Ax (green) to quantify DNA double strand breaks. Green γH2Ax were quantified in relation to total cell count by DAPI. (B) Immunofluorescent staining of non-senescent (NS), replicative senescent untreated (RS untr) and replicative senescent treated (RS treated) cells for DAPI (blue) and HMGB-1 (green). Number of HMGB-1 positive cells was counted in relation to total cell count by DAPI. (PDF)

Publication Details

Journal
Figshare
Published
2025-02-28
DOI
https://doi.org/10.1371/journal.pone.0319381.s003
Primary Topic
Mycorrhizal Fungi and Plant Interactions
Type
article
Field-Weighted Citation Impact
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article

The treatment with VPA, Li2CO3, and tranilast reduces DNA double strand breaks as indicated by γH2Ax but does not restore HMGB-1 levels.

Figshare
Mycorrhizal Fungi and Plant Interactions
article

The treatment with VPA, Li2CO3, and tranilast reduces DNA double strand breaks as indicated by γH2Ax but does not restore HMGB-1 levels.

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Abstract

(A) Immunofluorescent staining of non-senescent (NS), replicative senescent untreated (RS untr) and replicative senescent treated (RS treated) cells for DAPI (blue) and γH2Ax (green) to quantify DNA double strand breaks. Green γH2Ax were quantified in relation to total cell count by DAPI. (B) Immunofluorescent staining of non-senescent (NS), replicative senescent untreated (RS untr) and replicative senescent treated (RS treated) cells for DAPI (blue) and HMGB-1 (green). Number of HMGB-1 positive cells was counted in relation to total cell count by DAPI. (PDF)

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Mycorrhizal Fungi and Plant Interactions
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The treatment with VPA, Li<sub>2</sub>CO<sub>3</sub>, and tranilast reduces DNA double strand breaks as indicated by γH2Ax but does not restore HMGB-1 levels. · Figshare (2025) | TGRS Research Map | TGRS