Imaging parameters.

<p>Imaging parameters are listed by experiment type with relevant figures indicated. Imaging parameters were held constant across experiments with occasional exceptions. Where multiple settings were used, the most common are in bold. For spindle pole and mitotic error tracking, variation in imaging parameters was within the range commonly used by our lab and below the threshold for phototoxicity as determined in [<a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1011351#pgen.1011351.ref024" target="_blank">24</a>]. For experiments quantifying fluorescence intensity, data were normalized to the same point of reference to permit comparison across experiments.</p> <p>(DOCX)</p>