In vitro differentiation of EGS strain at 33°C.

Alive fluorescent imaging of EGS parasites detecting mCherry-expressing tachyzoites or GFP-expressing bradyzoites. (A) Representative fluorescent images of HFF cells infected with EGS parasites at 3 days in differentiation media at 37°C, showing mCherry-expressing tachyzoites. (B) Representative fluorescent images of HFF cells tracking the differentiation of EGS parasites into GFP-expressing bradyzoites under our differentiation protocol at 33°C from one to nine days post-infection. (PDF)