Tissue-specific targeting of <i>mif</i> in epithelial stem cells.

<p>(A) A map of the genetic Tol2 construct used to delete <i>mif</i> in epithelial stem cells. A U6 promoter was used to drive the expression of 2 different sgRNAs targeting <i>mif</i> in epithelial stem cells expressing Gal4. (B) A schematic of the ~1 kb deletion that occurs with CRISPR/Cas9 editing of the <i>mif</i> gene. (C) A gel comparing the PCR results between WT, <i>UAS</i>:<i>Cas9-T2A-GFP;mifsgRNA</i> and the <i>mif</i> stable mutants.</p> <p>(PDF)</p>