Loss of <i>fshr-1</i> has minimal effects on synaptic vesicle or active zone protein localization in GABAergic motor neurons.

<p>(A) Wild type worms and <i>fshr-1</i>(<i>ok778</i>) mutants that also expressed GFP::SNB-1 in GABAergic (GABA) neurons were imaged using a 100x objective. (<i>Left panel</i>) Representative images of the dorsal nerve cords halfway between the vulva and the tail of young adult animals. (<i>Right panels</i>) Quantification of puncta (synaptic) intensity and puncta density (per 10 𝜇m) ± s.e.m for n = 25 wild type and n = 31 <i>fshr-1</i>. Puncta intensity is shown normalized to wild type. (B-C) Wild type or <i>fshr-1</i>(<i>ok778</i>) mutant animals that also expressed (B) mCherry::UNC-10 or (C) GFP::SYD-2 in GABAergic neurons were imaged using a 100x objective. (<i>Upper panels</i>) Representative images of the dorsal nerve cords halfway between the vulva and the tail of wild type and <i>fshr-1</i> young adult animals. (Lower panels) Quantification of puncta (synaptic) intensity and puncta density (per 10 𝜇m) ± s.e.m. Puncta intensity is shown normalized to wild type. For (B), n = 26 for wild type, n = 27 for <i>fshr-1</i>. For (C), n = 17 for wild type, n = 20 for <i>fshr-1</i>. Student’s <i>t</i> tests were used to compare the means of the datasets. *<i>p</i> ≤ 0.05 are shown.</p> <p>(PDF)</p>