Phenotypes and structural analyses of uncharacterized A. baumannii genes.

Heatmaps of CG scores for clusters containing (A) GO593_00140 or (B) GO593_11615. Y-axis clustering was conducted using the Ward method and Canberra distance across all library knockdowns. (C) Heterodimer structural prediction (Alphafold2) for GO593_00140 and the chi subunit of DNA Polymerase III in <i>A. baumannii</i> (left) and the solved structure for the <i>E. coli</i> psi-chi subunit heterodimer (right). GO593_00140 has a lengthy unstructured N-terminal end absent in <i>E. coli</i> HolD. (D) Domain architecture map from InterProScan analysis, comparing <i>E. coli</i> LexA to GO593_11615. Both have a DNA-binding domain and S24 peptidase/LexA domain but differ in overall protein length and specific type of DNA-binding domain. (E) Domain architectures for <i>E. coli</i> FtsN and <i>A. baumannii</i> GO593_07290. GO593_07290 lacks similarity to the short ^EFtsN region required for function (47). BLAST-aligned amino acid sequences (24% identity) are depicted with dotted lines. (PDF)