Motor impairment in <i>cpx-1</i> was exacerbated in <i>cpx-1; unc-31</i> double mutants.

(<b>A</b>) Motility measured by thrashing number per minute in wild-type, <i>unc-31(e928)</i>, <i>cpx-1(ok1552)</i>, <i>cpx-1(ok1552); unc-31(e928)</i>, <i>cpx-1(Δ12)</i>, and <i>cpx-1(Δ12); unc-31(e928)</i> worms. <i>n</i> ≥ 39 animals. (<b>B</b>) Quantification of locomotion velocities in each genotype. <i>n</i> ≥ 39 animals. One-way ANOVA was performed (F_{(5, 230)} = 673.8, <i>P</i> < 0.0001 for <b>A</b>; F_{(5, 263)} = 566.6, <i>P</i> < 0.0001 for <b>B</b>), *** <i>P</i> < 0.001. The data are presented as the means ± SEMs. <i>N</i> = 3 independent replicates. All the raw data associated with this figure are available in <a href="http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.3003023#pbio.3003023.s016" target="_blank">S1 Data</a>. (PDF)