Target pathway effects for tetracycline-class antibiotics.

(A) Sina plots depict differential guide CPMs for ribosome genes (STRING identifier CL:113) in minocycline, tetracycline, or tigecycline compared to no chemical control. Chemical-gene interactions are described above each graph—up (positive), down (negative), or no change—with FDR values. Guides are weighted in this calculation by predicted efficacy, with perfect guides at 100. (B) Heatmap shows relative fitness scores(median log₂ fold changes) of ribosomal gene knockdowns of the mock treatment control compared to T0. Ribosome knockdowns all have substantial loss of fitness without additional chemical treatment. (C) Volcano plots of library gene CG scores (median log₂ fold change compared to mock treatment) in minocycline, tetracycline, and tigecycline. Genes in the ribosome group are colored green. Lines depict cutoffs for significance (|median log₂ fold change| ≥1, Stouffer’s <i>p</i> < 0.05). (PDF)