The effect of RNase III treatment on PIP2 and SON localization in non-permeabilized cell nuclei visualized by immunofluorescence staining.

<p><b>(A)</b> U2OS cells were treated by RNase III without a semi-permeabilization step, and subsequently stained with PIP2 and SON-specific antibodies. Images were acquired by fluorescence microscopy. <b>B)</b> Quantification of normalized mean PIP2 signal intensity levels after RNase III treatment in segmented nuclei for nuclear speckles (Sp) and nucleoplasm (Np) regions without semi-permeabilization step (orange bars) compared to mock and RNase III-treated semi-permeabilized U2OS cells (<a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1011462#pgen.1011462.g001" target="_blank">Fig 1C</a>). Scale bars correspond to 5 μm. Statistical analysis was performed using Student’s t-tests. Error bars correspond to SEM (**** P < 0.0001), n = 3, N = 76 mock-treated cells, N = 89 RNase III-treated semi-permeabilized cells, N = 58 RNase III-treated non-permeabilized cells.</p> <p>(PDF)</p>