DDT/ FDT transition in conjugations with plasmid pOX38 and R64 on solid media [Dataset]

Transconjugants per donor ([T]/[D], y axis) measured at different Recipient densities ([R] ≈ [N], x axis) in E. coli BW27783 mating on solid LB-agar surfaces, 1 Donor to 100 Recipients and 1 h. conjugation time for plasmids pOX38 (IncFI) and R64 (IncI1). As in Fig 1 in the main section, the black line represents the ideal DDT regime, where different kon produce different y-intercepts. The red line represents the FDT regime, where the conjugation efficiency is just 1/τ and the dotted curve corresponds to the fitting to Eq.3. Every black dot corresponds to the average of 3 technical replicates.