Different genetic cross schemes for gene trap swapping by RMCE.

The most effective cross scheme is scheme 1, in which the males are double homozygous for the cGAL gene trap (cGAL GT) and the germline-expressing Flp transgene (<i>bqSi711</i>). Hygromycin serves as a positive selection marker for both the successful RMCE single-copy insertion as well as the array with the RMCE donor constructs; histamine serves as a negative selection marker for the array. Refer to see <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1011541#sec015" target="_blank">Materials and Methods</a> for more details. (PDF)