Positive and negative controls for FACS sorting of folded vs. unfolded peptides.

<p><b>(A) YSD.</b> Yeast cells with no expression (Neg., black line), or expressing peptide 5JI4 (“positive”; green line), 5ji4CtoA (no disulfide bond control; orange line), 5ji4N (Alanine substitution of key residues; red line), or SFS (linker control; blue line) resulted in different distribution pattern in flow cytometry histograms. The grey arrow bar indicated the gating used for FACS sorting. <b>(B) BiFC.</b> <i>E</i>. <i>coli</i> cells harboring the empty vector (Neg., black line), pBAD-YN/TC (“background”; gray line), pBAD-5ji4CtoA (no disulfide bond control; orange line), pBAD-5ji4N (Alanine substitution of key residues; red line), pBAD-SFS (linker control; blue line), and pBAD-YN5ji4TC (“positive”; green line) are well-separated in a flow cytometry histograms. <b>(C)</b> Sequences of control peptides and mean fluorescence for each construct measured using YSD and BiFC.</p> <p>(PPTX)</p>