Effect of environmental conditions and protein expression on conjugation rates [Dataset]

<b>a)</b> Effect of temperature on the conjugation rate. Conjugation assays were performed as described in Materials and Methods. (1 h, 37 ºC, 1D:100R). Each point corresponds to an independent experiment <b>b)</b> Effect of media composition on the transfer rate for R388 conjugation and mobilization (W-wire) and pKM101 conjugation (LBagar: autoclaved LBagar, LBagar pH7.4: PBS-buffered at pH 7.4 and TB agar: Terrific Broth agar. <b>c)</b> Overexpression of <i>tra</i> genes does not increase the transfer rate. On the x axis, genes overexpressed in R388-carrying donors using a pBAD33 vector. On the y axis, the fold change between the conjugation frequency (as [T]/[D]) obtained overproducing each of the constructions shown in the figure, and the conjugation frequency obtained with an empty pBAD33 vector. Statistical significancy was determined using Welch’s corrected ANOVA test for samples with unequal variances.