Identification and antagonistic mechanism of strains against Staphylococcus aureus

In this study, using a strain tz-6 with antagonistic effect on Staphylococcus aureus as the research object, the strain tz-6 was identified by morphological observation, physiological and biochemical tests and 16S rDNA sequence analysis, and the effects of the strain tz-6 on the growth of S. aureus, β-galactosidase, deoxyribonucleic acid (DNA) and protein leakage degree, cell membrane formation and pyruvate release were investigated to explore its antibacterial mechanism. The results showed that strain tz-6 was identified as Streptomyces sp., the fermentation crude extract of strain tz-6 could damage the membrane and internal structure of S. aureus, increase the membrane permeability, leading to acidic substances and β-galactosidase release, enhance nucleic acid and protein content in bacterial supernatant, and hinder the body energy and material metabolism in S. aureus.