Quantifying\nPhospholipids in Organic Samples Using\na Hydrophilic Interaction Liquid Chromatography–Inductively\nCoupled Plasma High-Resolution Mass Spectrometry (HILIC-ICP-HRMS)\nMethod

In this study, a novel method using hydrophilic interaction\nliquid\nchromatography (HILIC) coupled with inductively coupled plasma high-resolution\nmass spectrometry (ICP-HRMS) was introduced for the quantification\nof phospholipids in oil samples. The method employed a bridged ethyl\nhybrid (BEH) stationary phase HILIC column with a tetrahydrofuran\n(THF)/water mobile phase, enhancing the solubility and detection of\nphospholipids. During the study, a gradient/matrix effect on ICP-HRMS\nsensitivity was observed and successfully compensated for experimentally,\nensuring reliable quantification results. This approach has proven\neffective for a wide range of different oil samples including vegetable\noils, animal fats, and phospholipid supplements. Notably, this method\nallowed the direct quantification of phospholipids in oil samples,\nbypassing the need for prior sample preparation methods, such as solid\nphase extraction (SPE), thereby streamlining the analytical process.\nThe precision, accuracy, and reduced need for extensive sample preparation\noffered by this method mark a significant advancement in lipids analysis.\nIts robustness and broad applicability have substantial implications\nfor industries such as food and renewable energy production, where\nboth efficient and accurate lipid identification and quantification\nare crucial.