Water-Soluble Fluorescent\nSensors for Quantification\nof Trace Cisplatin in Body Fluids from Clinical Cancer Patients

Accurate quantification of cisplatin (cDDP) in body fluids\n(blood,\nurine, and ascites) is crucial in monitoring therapeutic processes,\nassessing drug metabolism, and optimizing treatment schedules for\ncancer patients. Nonetheless, due to the inherent fluorescence and\ncomplexity of the body fluid matrix, along with the low cDDP concentrations\nin these fluids during treatment, using fluorescent sensors for fluid\ndetection remains a subject of ongoing research. Herein, a series\nof water-soluble cDDP-activatable fluorescent sensors was rationally\nconstructed by introducing thioether groups to the xanthene skeleton\nbased on the chalcogenophilicity of platinum. These sensors exhibit\nexcellent sensitivity and certain anti-interference capabilities for\nsensing cDDP in living cells, rat tissues, and zebrafish. Especially,\nwith a simplified sample pretreatment procedure, for the first time, <b>Rh3</b> and <b>Rh4</b> have enabled quantitative detection\nof cDDP levels in diversiform body fluids from clinical ovarian and\nbladder cancer patients. These results are highly consistent with\nthose obtained by ICP-MS detection. This work paves the way for utilizing\nfluorescent sensors in clinical body fluid analysis, thus potentially\nrevolutionizing the monitoring methods of cDDP in clinic settings.