Zeolitic Imidazolate\nFramework‑8 Nanocomposite\nHydrogels for Diabetic Wound Healing

To avoid the problem of difficult wound healing caused\nby diabetes.\nIn this study, zeolite imidazolium ester skeleton-8 (ZIF-8) was loaded\nwith dihydroquercetin (DHQ) in order to improve the slow-release property\nas well as the biological activity of DHQ, and the mechanism of wound\nhealing action of ZIF-8-loaded hydrogel was investigated by Western\nblot technique. The results showed that the dynamic light scattering\nresult was 90.54 ± 3.45 nm and the zeta potential was −32.91\nmV. In addition, through the synergistic effect of ZIF-8 and DHQ,\nthe hydrogel exhibited excellent in vitro activity, and the in vitro\nantioxidant results showed that the inhibition rate of DZP hydrogel\nagainst the free radicals of ABTS and DPPH was over 90%, and the inhibition\nrate against Escherichia coli and Staphylococcus aureus was over 90%, respectively,\nand also promoted cell growth and migration. In addition, the in vitro\nrelease assay of DHQ from DZP hydrogel at 24 h showed 61.69 ±\n1.93% at pH = 6.0 and 62.42 ± 1.58% at pH = 7.4, which improves\nthe slow-release property of DHQ and is suitable for long-term tissue\nrepair applications. The results of in vivo experiments showed that\nDZP hydrogel promotes the healing of difficult-to-heal wounds by inhibiting\nthe activation of the IkBα/NF-κB pathway, stimulating\nskin neovascularisation, and promoting collagen accumulation. Collectively,\nthese properties make DZP a strong candidate for diabetic wound dressings\nand are expected to play an important role in wound management.