Method validation and assessment of fatty acid content in variant types of durian (

\nDurian seeds are often considered useless, but they contain fatty acids that are rich in nutrients. Therefore, this study aims to determine the validity of the test method for the content of fatty acids found in variant types of durian seeds (\nDurio zibethinus Murr) in the Bangka Belitung region using GC-MS. Validation of the GC-MS method was carried out with palmitic, stearic, and oleic acid standards by assessing different parameters, namely system suitability, linearity, selectivity, accuracy, and precision with the gas chromatography method. The derivatization using Soxhlet with n-hexane and BF3-Methanol. The method validation showed that the gas chromatography method met all the parameters for each fatty acid in the form of palmitic, oleic, and stearic acids with a selectivity Rs, linearity, LoD, LoQ, RSD, and K’. Additionally, the 3 types of durian seeds contain fatty acids in the form of palmitic, oleic, stearic, and linoleic acids. The composition of palmitic acid in Tembaga, Bangkok, and Sijantung seeds was 5.160 ± 0.080, 5.057 ± 0.132, and 4.216 ± 0.099 w/w. The stearic acid content in 3 variant types of Tembaga, Bangkok, and Sijantung durian seeds include 3.182 ± 0.027, 3.755 ± 0.098, and 2.670 ± 0.032 w/w. Keywords: Durian seed; fatty acid content; gas chromatography; validation.\n