Defending Ti6Al4Vagainst Biofilm Formation with AlbuminBiofunctionalization

Surface biofunctionalization with structurally perturbed albumin, as well as with other plasmatic proteins, inhibits the initial bacterial adhesion and biofilm formation, involved in numerous healthcare-associated infections. In fact, we have reported this protective effect with thermally treated plasmatic proteins, such as albumin and fibrinogen, adsorbed on flat silica surfaces. Here, we show that albumin biofunctionalization also works properly on flat Ti6Al4V substrates, which are widely used to fabricate medical devices. The protective effect is conserved even in biologically relevant fluids, containing other proteins that potentially adsorb onto and/or displace preadsorbed albumin from the biofunctionalized substrates. We further demonstrate that the presence of structurally perturbed albumin on the substrate does not trigger macrophage activation and the release of inflammatory mediators. Consequently, surface biofunctionalization with thermally perturbed albumin is a simple strategy to prepare antibacterial, nonimmunogenic medical devices.