Detoxification\nof Chlorfenapyr by a Parkin-GSTd2 Module\nin <i>Bactrocera dorsalis</i> (Diptera: Tephritidae)

The oriental fruit fly, Bactrocera dorsalis (Hendel), is a highly invasive\nand destructive pest. Chlorfenapyr\nis a widely used insecticide that disrupts mitochondrial activity.\nThe Parkin protein plays conserved roles in maintaining mitochondrial\nhomeostasis, but the role of Parkin in response to chlorfenapyr remains\nlargely unknown. Here, we report that BdParkin is required for chlorfenapyr\ndetoxification, and dsRNA targeting <i>BdParkin</i> improves\nthe insecticidal efficacy of chlorfenapyr. Among the genes whose expression\nlevels are affected by <i>BdParkin</i> RNAi, knock-down\nof the glutathione S-transferase gene <i>BdGSTd2</i> increases\nthe insecticidal efficacy of chlorfenapyr. Molecular docking reveals\npotential interactions between BdGSTd2 and tralopyril, an insecticidal\nmetabolite of chlorfenapyr. These results suggest that BdParkin could\nimpact the response of B. dorsalis to\nchlorfenapyr through metabolic processes regulated by BdGSTd2. Our\nfindings could offer new insights into how insects detoxify chlorfenapyr\nand provide molecular targets for developing a sustainable management\nstrategy for B. dorsalis.