Size Matters:\nCurvature and Antigen-Mediated Dual\nRecognition of Size-Specific Tumor-Derived Exosomes

Accurate identification of tumor-derived exosomes is\ncrucial for\nadvancing cancer diagnosis and therapies. However, distinguishing\ntumor-derived exosomes is challenging due to the heterogeneity of\nexosomes, which reflect different sizes and cells of origin. To address\nthis challenge, we introduce the <b><u>c</u></b>urvature and <b><u>a</u></b>ntigen-mediated <b><u>p</u></b>roximity ligation assay for <b><u>tu</u></b>mo<b><u>r</u></b>-derived <b><u>e</u></b>xosomes (<b>CAPTURE</b>) strategy, which leverages the size-selective properties of curvature-sensing\npeptides and specific antigen binding of aptamers. CAPTURE enables\nhighly specific identification and precise quantification of the PD-L1<sup>+</sup> exosomes in plasma samples. CAPTURE is proven to be simple,\nhomogeneous, rapid, and highly selective, achieving a 100% specificity\nin discriminating colorectal cancer (CRC) patients from healthy donors.\nOverall, the CAPTURE strategy presents a promising avenue for precise\nand noninvasive cancer diagnosis.