Fabrication and\nCharacterization of Gelatin-Finger\nCitron Polysaccharide Nanoparticles for Enhanced Solubility and Bioavailability\nof Luteolin in Treating Acute Alcoholic Liver Disease

Luteolin (Lut) is a natural flavonoid that has been widely\nused\nin the treatment of liver and glycolipid metabolic diseases. However,\npoor water solubility and bioavailability limit its efficacy and application.\nTo overcome these challenges, a protein–polysaccharide composite\nnanoparticle (L-GF NPs) for Lut delivery was prepared by a self-assembly\nmethod based on amphiphilic gelatin and active finger citron polysaccharide\n(FCP). The L-GF NPs were approximately spherical in shape and were\nformed by hydrogen bonding, electrostatic interaction, and hydrophobic\ninteraction. Lut was encapsulated in the nanoparticles in an amorphous\nstate, and the encapsulating efficiency was 79.49%. The efficacy of\nL-GF NPs was evaluated by intragastric administration of an alcoholic\nliver disease (ALD) mouse model. L-GF NPs have been demonstrated to\nsignificantly ameliorate the symptoms of ALD mice, which are mainly\nachieved by reducing lipid accumulation and oxidative stress and inhibiting\nthe NF-κB pathway. In conclusion, the aqueous solubility and\nbioavailability of Lut were markedly enhanced by FCP encapsulation.\nMeanwhile, the combined effect of Lut and FCP significantly ameliorated\nALD and attenuated alcohol-induced lipid metabolic disorders and oxidative\ndamage. This provides a new strategy to promote the prevention and\ntreatment of ALD.