Green Tea Diet\nCan Effectively Antagonize the Toxicity\nInduced by Environmental-Related Concentrations of BPA: An Implication\nfrom <i>In Vivo</i> and <i>In Silico</i> Studies

The neurotoxicity of bisphenol A (BPA) exposure has been\nconfirmed <i>in vitro</i> and <i>in vivo</i>,\nand inflammatory\nresponse is considered the main pathway. Green tea is a healthy life\nhabit as it is rich in various anti-inflammatory components. To confirm\nthat green tea diet is an effective measure to antagonize BPA-induced\nneurotoxicity, mice were treated with 0.5 and 5000 μg/kg/day\nof BPA from postnatal days (PNDs) 10–50 and supplemented with\ngreen tea on PND 21. From PND 51, behavioral tests were conducted\non mice to assess their emotional, cognitive, and spatial learning\nmemory capabilities. The open field test and elevated plus maze test\nindicated anxiety-like behaviors induced by BPA. Interestingly, green\ntea diet significantly alleviated BPA-induced anxiety-like behaviors.\nMeanwhile, the green tea diet effectively reversed BPA-induced microglia\nactivation and morphological changes in the hippocampus of mice. Molecularly,\ngreen tea inhibited hippocampal neuroinflammation of mice by reducing\nBPA-induced expressions of NLRP3, ASC, cleaved-caspase-1, GSDMD-N,\nIL-6, and IL-1β, as well as significantly reducing the expression\nof Bak1, Bax, caspase-9, and Cytc c genes (<i>p</i> <\n0.05). Molecular docking suggests that various anti-inflammatory components\nof green tea can competitively bind to the estrogen receptors with\nBPA. In general, a green tea diet alleviates BPA-induced emotional\ndisorders by inhibiting microglial polarization and hippocampal pyroptosis,\nindicating its effective antagonistic ability against the neurotoxicity\ninduced by environmental BPA exposure.