Investigation of\nHost–Guest Inclusion Complex\nof Mephenesin with α‑Cyclodextrin for Innovative Application\nin Biological System

The goal of this study was to use coevaporation to look\ninto how\npolyether compounds like mephenesin (MEP) can be encapsulated into\nthe host molecule α-cyclodextrin’s nanohydrophobic cage.\nFourier transform infrared spectroscopy (FT-IR) investigations, powder\nX-ray diffraction (PXRD), and <sup>1</sup>H NMR were among the spectroscopic\ntechniques used to describe the inclusion complex. Additionally, Job’s\nplot has been utilized to illustrate how MEP is encapsulated with\nα-cyclodextrin (α-CD) at a 1:1 molar ratio. The thermal\nstability of MEP increased after encapsulation according to thermogravimetric\nanalysis (TGA) and differential thermal analysis (DTA) experiments.\nMephenesin fits into the cavity of α-cyclodextrin in a 1:1 ratio,\nas observed by molecular docking for the inclusion complex to find\nthe most appropriate orientation. This observation is further supported\nby the Job plot. Furthermore, a comparison was carried out based on\na cell viability study between the medication and its inclusion complex.