Additional file 3 of Analysis of early childhood intestinal microbial dynamics in a continuous-flow bioreactor

Supplementary Material 3: Figure 2. Five additional fecal samples cultured in the chemostat model generate bacterial communities representative of the donor’s microbiome.Relative abundances of amplicon sequence variants (ASV) that represent bacterial composition were identified by Illumina MiSeq 16S rRNA gene sequencing from fecal samples and longitudinal chemostat communities of an additional five healthy 18-24-month-old participants. (a‑j) Alluvial plots show the relative abundance of ASVs identified on the fecal sample (day 0) and the chemostat culture over time from S2 (a, f), S3 (b, g), NS4 (c, h), S5 (d, i) and NS6 (e, j) individuals. (a-e) ASVs, indicated by colors, are stratified by presence or absence in on the indicated days of culture. The relative abundance of ASVs from the fecal sample found in the chemostat culture is indicated in brackets adjacent to the day 0 bar. (f-j) Colors represent the nine predominant phyla. ASVs are ordered by prevalence categories. (k) Bray-Curtis dissimilarity PCoA of (day 0) fecal sample and day 17 chemostat samples from seven study participants. The first and second principal coordinates are shown. Values in the brackets indicate the % of total variability explained by each principal coordinate. Point shape indicates sample type, and point color indicates the source individual.